Figure 2. Apoptotic cell death was observed in LLC (A) and B16F10 (B) cell lines. Cells were treated for 18 h with the indicated Rg3 (100, 300, 500 µg/ml) and Doxorubicin (0.01 µg/ml) concentrations. The percentage of apoptotic cells were determined by measuring Annexin V/PI expression using flow cytometry. Each experiment was done in triplicate. Significant effects versus control are indicated with asterisks (*p<0.05, **p<0.01); # indicates the significant changes (#p<0.05) compared to Doxorubicin (0.01µg/ml).
