Figure 5. (A) LLC was incubated overnight with or without doxorubicin or Rg3 at 37℃. LLCs were then stained with CRT-FITC. DCs were labeled with CD11c-PerCP5.5 then co-cultured with CRT-FITC labeled LLC for 6 h. FITC and PerCP5.5 double stained cells were considered as DCs taken tumor cells. Each experiment was done in triplicate. (B) B16F10 was incubated overnight with or without doxorubicin or Rg3 at 37℃. B16F10s were then stained with CRT-FITC. DCs were labeled with CD11c-PerCP5.5 then co-cultured with CRT-FITC labeled B16F10 for 6 h. FITC and PerCP5.5 double stained cells were considered as DCs taken tumor cells. Each experiment was done in triplicate. Significant effects versus control are indicated with asterisks (*p<0.05); #indicates the significant changes (#p<0.05) compared to Doxorubicin (0.01µg/ml).
