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. 2016 Feb 23;20(2):177–183. doi: 10.4196/kjpp.2016.20.2.177

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Copyright © Korean J Physiol Pharmacol

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Activity-driven isolation of K49-PLA2 from crudePDE I (C. atrox venom)was done using a three-step chromatography procedure as described in Materials and Methods. (A) Semi-preparative reverse phase C-18 (20×150 mm) column recycling preparative HPLC. (B) Size exclusion HPLC of fraction 10 from semi-preparative reverse phase HPLC. (C) Reverse phase chromatography of the fraction 5 from size exclusion HPLC.