Fig. 5. K49-PLA2 had no cytotoxic effect on neutrophils.

LDH was measured according to LDH measuring kit (Takara) protocol after 1 h-incubation of neutrophils with K49-PLA2 (1 µg/ml). Absorbance was read at 490 nm with fluorescence micro plate reader (SPECTRAmax M2e, Molecular Devices). Medium alone and medium from cells incubated with 0.1% (v/v) of Triton X-100, were used as reference controls for 0% and 100% cytolysis, respectively.