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. 2016 Feb 23;20(2):221–228. doi: 10.4196/kjpp.2016.20.2.221

Fig. 4. Effect of prunetin on the gene expression of MMP-1, MMP-13, ADAMTS-4, or ADAMTS-5 in rabbit chondrocytes.

Fig. 4

Primary cultured rabbit articular chondrocytes were pretreated with varying concentrations (1, 10, 50, and 100 µ M) of prunetin for 2 h and then stimulated with IL-1β (10 ng/mL) for 24 h. The gene expression level of MMP-1, MMP-13, ADAMTS-4, or ADAMTS-5 was measured by RT-PCR. Three independent experiments were performed and the representative data were shown. The signal intensity of each band was analyzed by GelQuant software (DNR Bio-Imaging Systems Ltd., Jerusalem, Israel). Each bar represents a mean±S.E.M. of three independent experiments in comparison with that of the control set at 100%. *significantly different from control (p<0.05), +significantly different from IL-1β alone (p<0.05) (cont: control, concentration unit is µM).