Figure 4.

Increased NF-κB activity in Jurkat-Tat cells was dependent on PKCθ activation. Binding of p65/RelA to κB consensus sites in Jurkat-Tat cells treated or not with PMA was analyzed by DAI assay (A). The same experiment was performed in Jurkat-Tat cells transfected with siRNA directed against mRNA for PKCθ. Analysis by immunoblotting of the level of PKCθ interference is shown (B). Densitometry analysis was done to calculate relative p65/RelA expression and showed as horizontal bar diagrams (A,B). Interference of mRNA for PKCθ in Jurkat-Tat cells transfected with a luciferase expression vector under the control of LTR promoter (pLTR-LUC) was analyzed by chemiluminescence. Data are represented as mean ± SEM. *** for p < 0.001 (C).