Figure 7.

Nuclear coexistence of PKCθ and Tat in Jurkat cells and human PBLs. Nuclear proteins from Jurkat E6-1 cells transiently transfected with pCMV-Tat101 for 48 h or from PBLs infected with NL4-3_wt for 3 days were analyzed by sequential ChIP assay to detect the interaction between PKCθ and Tat in the HIV-1 LTR promoter, specifically in the region from −39 to +66 that contains the TAR loop (+1 to +59). ChIP enrichment ratio of two independent experiments is represented. Data are represented as mean ± SEM. * and ** for p < 0.05 and p < 0.01, respectively. Schematic representation of the proximal HIV-1 LTR promoter show the three binding sites for Sp1 and the two binding sites for NF-κB, located upstream TAR loop.