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. 2015 Oct 22;44(4):1541–1552. doi: 10.1093/nar/gkv1088

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Contribution of upstream elements on topoI promoter activity. (A) Schematic representation of the constructs generated for the study. (B) Measurement of the activity of various constructs harboring topoI promoter(s) by β-galactosidase assay. (C) Determination of gyrase binding on upstream region of topoI by ChIP-qRT PCR using the primers specific to the topoI upstream region. The enrichment values represent the enrichment of DNA fragment of interest (topoI promoter region and ORF) in immunoprecipitated (IP) sample over the mock. The error bars represent the SD obtained from three independent experiments.*P < 0.01, **P < 0.001, ***P < 0.0001, ns: not significant (P > 0.1).