Figure 4.

Identification of Transcription Start Sites of topoI gene from M. smegmatis. (A) Primer extension analysis was carried out to map the TSS upstream of topoI as described in Materials and Methods. The primer extension products corresponding to the transcription start site for each promoter is indicated (arrows). E: exponential phase culture without novobiocin treatment, N: exponential phase culture treated novobiocin (100 μg/ml) for 3 h, S: stationary phase culture without novobiocin. (B) Table representing the 5′ UTR length and first nucleotide of the each transcripts.