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. 2015 Oct 22;44(4):1541–1552. doi: 10.1093/nar/gkv1088

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Supercoiling sensitive expression of topoI is specific to native topoI promoter(s). (A) Promoter elements upstream to the start sites. Arrows indicates the transcription start sites. (B) Schematic showing the replacement of native promoter(s) of topoI with a ptr promoter (having optimally spaced −35 and −10 elements—17 bp spacer). (C) Immunoblot analysis of the TopoI and gyrase expression in native M. smegmatis cells and mutant strain with ptr promoter. The exponential phase recombinant and WT M. smegmatis cells were treated with novobiocin (100 μg/ml) for 6 h and processed for the immuno-detection of TopoI.