Figure 6.

Factors involved in CenH3 loss. Immunostaining with CenH3a antibody at late stages of MAC development following control (Paramecium fed with E. coli producing dsRNAs corresponding to the plasmid L4440 with no sequence target in the Paramecium genome), PGM, EZL1, DCL2 and DCL3 or DCL5 RNAi. Schematic representations of cells are presented on the left. Dashed circles indicate the two new developing MACs and filled arrows indicate the two MICs. Scale bar is 10 μm. Quantification of the number of cells with CenH3a positive signal in the new developing MAC was performed for at least 100 cells for each RNAi condition in two or three independent experiments. Early stages of MAC development from the same experiments are presented on Supplementary Figure S6.