Figure 1.

Disorder in Mediator complex subunits of metazoans, plants and fungi. (A) Average disorder of Mediator subunits in metazoans, plants and fungi. Error bars represent the standard deviation in the average disorder. The subunits belonging to different modules (Head, Middle, Tail and Kinase) have been separated by vertical dashed lines. 0.5 (dashed line) is the threshold for disorder of the subunit. Significant differences as determined by Mann–Whitney test at alpha level 0.05 are indicated by (.) for metazoan versus plants, (*) for metazoans versus fungi and (#) for plants versus fungi. (B) Clustering of metazoans, plants and fungi based on the average disorder of the Mediator subunits. Euclidean distance between any two organisms was calculated using average disorder of the corresponding subunits to make a 146 × 146 distance matrix. Unrooted tree was constructed from the distance matrix using PHYLIP program by the neighbor-joining method. Lower organisms of all the three kingdoms metazoans, plants and fungi cluster in Group I. Fungi (blue), plants (green), lower metazoans (magenta) and higher metazoans (red) segregate into Group II, Group III, Group IVa and Group IVb, respectively. (C) Summary of the clustering of the major phyla and kingdoms.