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. 2015 Nov 20;44(4):1591–1612. doi: 10.1093/nar/gkv1135

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Characterization of AtMed4 as a hub subunit in Arabidopsis Mediator complex. (A) Clones obtained after Yeast two-hybrid screening using AtMed4 as bait. False positive clones were characterized by yeast two hybrid analysis using empty vector (BD) as a control. Cultures of co-transformed yeast clones adjusted to an OD of 0.2 were spotted on synthetic drop out medium SD-Trp⁻/Leu⁻/His⁻/Ade⁻ to score the interactions. Growth on synthetic double drop out (DDO) medium SD-Trp⁻/Leu⁻ media was used as control for proper growth. (B) The interactome of AtMed4 represents diverse proteins belonging to the categories of general transcription factors (yellow triangles), enzymes (diamonds), Mediator subunit (vee), ubinquitinylation proteins (octagon), kinase (hexagon) and other proteins (rectangles).