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. 2015 Nov 3;44(4):1613–1629. doi: 10.1093/nar/gkv1151

Figure 3.

Figure 3.

HspBP1 levels inversely correlate with HIV-1 virus production. (A) HspBP1 knockdown results in increased HIV-1 virus production in a dose dependent manner in HEK293T cells. Virus production was analyzed by p24 ELISA with culture supernatants from HEK293T cells co-transfected with pNL4-3 and increasing doses of HspBP1 siRNA. Supernatant was collected 48 h post-transfection. (B) HspBP1 silencing enhances virus production in HIV-1 infected Jurkat cells. Jurkat cells were transfected with HspBP1 siRNA followed by infection with HIV-1NL4-3 virus after 24 h. p24 ELISA was performed on the supernatant collected after 48 h of infection. (C) HspBP1 over-expression inhibits virus production in dose dependent manner in HEK293T cells. Culture supernatants of HEK293T cells transfected with pNL4-3 and increasing doses of HspBP1 vector were analyzed for virus production using the p24 antigen capture ELISA. (D) HIV-1 inhibition by HspBP1 is a specific occurrence as mutant failed to inhibit virus production. HEK293T cells were co-transfected with either wild type or mutant HspBP1 and pNL4-3. Cells were harvested 48 h post-transfection. Virus production was determined by p24 ELISA on culture supernatant. Silencing using siRNA or over-expression of HspBP1 was confirmed by immunoblotting in each experiment. The results are representative of three experiments and presented as the mean ± SD (*P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001).