Figure 5.

Influence of p400 depletion on the recruitment of HR and NHEJ factors at DSB. (A) Chromatin immunoprecipitation (ChIP) experiment performed on U2OS-AsiSI cells. DSBs were induced by OHTam addition and ChIP experiments were performed with Rad51 and XRCC4 antibodies. One representative experiment from three totally independent experiments is shown. For each conditions, three differents DSBs were examined together with the P0 as control. Mean and standard deviation have been calculated on the PCR values. (B) Generation of single strand DNA around DSB was evaluated in U2OS-AsiSI cells on two different DSBs. Cells were transfected with p400 siRNA and 48 h later DSBs were induced by OHT addition during 4 h. Typical experiment (from three independent experiment) is shown with mean and sd from PCR performed in triplicate.