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. 2015 Dec 31;44(4):1894–1908. doi: 10.1093/nar/gkv1522

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Construction of the KTA1493 strain. (A) Western blotting analysis. The expression of Ta1493 gene product was assessed by western blotting analysis. Proteins from the ΔarcTGT and KTA1493 strains cultured at 60 or 85°C were separated by 15% SDS-PAGE (left) and western blotting analysis was performed (right). (B) Nucleoside analysis in tRNA fractions from the KTA1493 strain, which was cultured at 60ºC (upper panels) and 85ºC (lower panels). 0.2 A260 units of tRNA fractions were analyzed by 10% PAGE (7 M urea) (left panels). The gels were stained with toluidine blue. Modified nucleosides in tRNA fractions were analyzed (right panels). (C) Nucleoside analysis in tRNA fraction from the KUWA strain, which was cultured at 60ºC. 0.2 A260 units of tRNA fractions were analyzed by 10% PAGE (7 M urea) (left panels). The gels were stained with toluidine blue. Modified nucleosides in the tRNA fraction were analyzed (right panel).