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. 2015 Dec 31;44(4):1894–1908. doi: 10.1093/nar/gkv1522

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — MALDI-MS analysis of the wild-type tRNA^Leu. (A) The wild-type tRNA^Leu was expressed in T. kodakarensis strain KUWA and purified as a control. The purified tRNA^Leu was digested with RNaseA and its fragments were then analyzed by MALDI-MS spectrometry. In this region, a fragment (m/z = 1713.2), which coincided with the expected m/z value of the G13-C17 fragment (GAG⁺ACp) in the wild-type tRNA^Leu, was detected. (B) The fragment sequence (m/z = 1713.2) was determined by MS/MS analysis as GAG⁺ACp. (C) The RNaseA-digested fragments derived from the wild-type tRNA^Leu expressed in the KTA1493 strain were analyzed by the same method as (A). In the 1600–1800 m/z region, a new fragment (m/z = 1754.3) appeared. (D) The sequence of this fragment (m/z = 1754.3) was determined as G⁺AG⁺ACp, which corresponded to the G⁺13-C17 fragment in the wild-type tRNA^Leu. (E) Two fragments (G⁺AGACp and GAG⁺ACp; m/z = 1713.2) were detected by MS/MS analysis.