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. 2016 Jan 14;44(4):1776–1788. doi: 10.1093/nar/gkv1538

Figure 1.

Figure 1.

Stability of RH monomer and dimer. (A) Incubation of purified RHmnel monomer for a period of ∼20 h at 25°C yielded only the monomer (red curve); incubation of purified RHmnel monomer at 37°C over a similar period yielded a monomer – dimer mixture (blue curve). The position of the purified dimer corresponds to the yellow curve. Fractions were separated on a Superdex 200 10/300 column. (B) Purified samples of RHΔNT monomer (gold curve) or dimer (green) rapidly reloaded on the Superdex 200 column HiLoad 26/60, eluted as monomer-dimer mixtures. Chromatogrpahic separations were run at 4°C using 50 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA as the running buffer.