| 5′-CATGTTGCCTGGTTGATGTGGTGA TATCACCGATTTATCCGTGTCTTAGA GGGACAATCGATGGTGAGCAAGGGCG AGGAGC-3′ |
5′-GGGCATCAAATGATGCCCTTTTAGTGCG CATTGCGTCAAATGTTATCGGCAACATT ACCCTGTTATCCCTAGTTAC-3′ |
venus primer with flanking tufB ORF homology sequences for RED E/T recombination |
| 5′-GTCTTCTTGCGCTAATTTTTTGCTAG GGATAACAGGGTAATTCTAGAGACGC ACACCGTGGAAAC-3′ |
5′-CATGGAATTCATTACCCTGTTATCCCTA TGGACTCACAAAGAAAAAACGCCCGGTG TGCAAGACCGAGCGTTCTGAACAACCCG GGGCGGCGTTGTGACAATTTAC-3′ |
Gentamycin resistance gene primer for overlap extension with tetP-mCherry |
| 5′-CATGAAGCTTGTTGACACTCTATCAT TGATAGAGTTATTTTACCACTCGGAT CCAGGAGGAACAATATGGTGTCTATC ACTAAAGATCAAATCATGGTGAGCAA GGGCGAGGAG-3′ |
5′-ATTACCCTGTTATCCCTAGCAAAAAATT AGCGCAAGAAGACAAAAATCACCTTGCG CTAATGCTCTGTTACAGGGTACCTTACTTG TACAGCTCGTCCATG-3′ |
tetP-mCherry primer for overlap extension with the Ampicillin resistance gene |
| 5′-GGGAGGAATAATAAGAAAAAATCT CTGCCAGGAAGCTATCGTTGAAAAGC AATGTGACGAGCGGATAACAATTTCA CACAGG-3′ |
5′-CACTTTTTCAACATCATTGTGCTCAACA ATGCGCTCCTGCTAAACCATAATTCTTTTT ATCAGAGGGTTTTCCCAGTCACGACGTT-3′ |
primer for amplifying the tetP-mCherry-Ampicillin construct with homology for a divergent insertion in the upstream region of tufB
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