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. 2016 Jan 26;44(4):1525–1540. doi: 10.1093/nar/gkw039

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — qRT-PCR analysis, predicted hairpin structure and genomic localization of putative novel retina miRNAs. (A) The expression of nine predicted novel miRNAs was validated by qRT-PCR on RNA from the human retina, RPE/choroid, brain, liver, muscle and blood. The retina was used as calibrator tissue. miRDeep output (predicted hairpin structure and associated score) (B, D) and genomic localization (C, E) of the putative novel miRNAs nrm7 and nrm29 that overlap with the transcriptional unit of WASF3 and OTX2, respectively.