Figure 5.

Left: PaeLigD is endowed with a 5′-dRP lyase activity. The assay was performed as indicated in Materials and Methods in the presence of either 3.5 nM of EndoIII or 60 nM of the indicated LigD in the absence (−) or presence (+) of 0.64 mM MnCl₂. After incubation during 30 min at 30°C samples were analyzed by 8 M urea-20% PAGE and autoradiography. Position of products is indicated. Alk, alkaline hydrolysis of the 5′-dRP moiety. Right: the 5′-dRP lyase activity of BsuLigD resides in the ligase domain. The assay was performed as in left panel in the presence of 216 nM LigDom. After incubation during 30 min at 30°C samples were analyzed by 8 M urea-20% PAGE and autoradiography. Position of products is indicated. The figure is a composite image made from different parts of the same experiment.