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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Arthritis Rheumatol. 2016 Feb;68(2):359–369. doi: 10.1002/art.39442

Figure 1. RPTPα is enriched in the RA synovial lining and promotes TNF and IL-1β signaling in RA FLS.

Figure 1

(A) Immunohistochemical staining of RA synovial sections using anti-RPTPα or control IgG antibodies. (B–C) RA FLS (n=4) were treated with 2.5 µM control non-targeting (Ctl) or PTPRA PMO for 7 d. (B) PTPRA mRNA expression levels were measured by qPCR. Median and interquartile range (IQR) is shown. *, p<0.05, Mann-Whitney test. (C) RPTPα protein levels were measured by Western blotting. (D) Following treatment with PMO, RA FLS (n=4) were stimulated with 50 ng/ml TNF or 2 ng/ml IL-1 for 24 hr. mRNA expression was analyzed by qPCR. Median and IQR is shown. Protein expression in cell supernatants was measured by ELISA. Mean ± standard error of the mean (SEM) is shown. *, p<0.05, Mann-Whitney test.

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