Figure 4. FAK inhibition impairs activation of JNK and TNF and IL-1β-induced gene expression in RA FLS.

(A) RA FLS (n=4) were stimulated with 50 ng/ml TNFα or 2 ng/ml IL-1β in the presence of DMSO, the FAK inhibitor PF573228, or the AKT inhibitor MK2206 for 24 hr. mRNA expression was analyzed by qPCR. Mean ± SEM is shown. *, p<0.05, Mann-Whitney test. (B) RA FLS were stimulated with 50 ng/ml TNF or 2 ng/ml IL-1β for 30 min or left unstimulated, in the presence of DMSO or 10 µM FAK inhibitor PF573228. Data is representative of 4 independent experiments.