Figure 3.

Reduced fat mass over time and improved insulin tolerance of ACSL5^−/−mice. ACSL5^−/− (KO) male mice and their littermate male ACSL5^loxP/loxP floxed control (FL) mice were fed a standard purified diet (see Methods). Shown (A, Body Composition) are body weights, lean and fat mass measured using magnetic resonance at 3, 4 and 6 months of age. Insulin tolerance showing mean ± SEM of raw (B) and percent (C) glucose response to 0.75 mg/kg of insulin administered intraperitoneally of four-month-old male mice were determined (see Methods, n = 8–10 mice per group). Each time point and for Insulin Tolerance Area Under Curve were compared by student's t-test between ACSL5^−/− (KO) and their littermate male ACSL5^loxP/loxP floxed control (FL) mice, significance is indicated by *(p < 0.05).