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. 2016 Jan 11;5(3):210–220. doi: 10.1016/j.molmet.2016.01.001

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Gene expression profile ACSL5^−/−knockout mice. Mean mRNA fold expression (see Methods) ± SEM of the liver, interscapular brown adipose tissue, and epididymal white adipose tissue of 5–7 mice were determined by semi-quantitative real-time RT-PCR, normalized to Cyclophyllin B, and normalized again to the mean result of each gene in floxed control ASCL5^loxP/loxP (FL) mice samples; * (p < 0.05) indicates significant difference between –δC_T values of ACSL5^loxP/loxP and ACSL5^−/− for each gene comparison.