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. 2016 Jan 15;5(3):198–209. doi: 10.1016/j.molmet.2016.01.003

Figure 4.

Figure 4

Delayed switch in leptin responses in a subset of NAG neurons from pups exposed to postnatal undernutrition. (A) Representative images of the ARH from leptin-treated offspring of control and large litters at P30 showing co-localization between Npy-GFP+ (green) and pSTAT3+ (upper panel, red) or c-Fos+ (lower panel, red) from mice of control or large litters at P30–35. Magnified views of the individual merged layers from the boxed areas in the composite are shown at the right. Scale bar = 100 μm 3V: Third ventricle. (B) Quantification of Npy-GFP+ neurons per ARH hemisection that co-stain for pSTAT3+ (upper panel) or c-Fos+ (lower panel) from offspring of control litters (CTL, white/black bars) or large litters (LL, blue bars) treated with saline (light colored bars) or leptin (4 mg/kg, i.p.; dark colored bars). Data are presented as mean ± s.e.m cell number/hemisection; n = 6–12 hemisections per mouse, 4–6 mice per age and group, 3 different litters; *p < 0.05 leptin vs saline, #p < 0.05 CTL vs LL, two-way ANOVA followed by Bonferroni post hoc test.