Figure 1.

(A) Gys1 targeting DNA construct (Ozgene), (B) The final MLC 1F-mercremer DNA transgenic construct. Expression of mercremer is driven by the MLC1F promoter. The MLC enhancer confers the tissue-specific expression. Two fragments of 5′ HS4 insulators were used to improve mercremer expression, (C) Last step of the breeding strategy to generate tamoxifen-inducible gys1 KO mice. gys1^lox/lox-mercremer^+/− mice were crossed with gys1^lox/lox mice. This breeding design can result in 4 different genotypes. The 2 genotypes of interest were selected for experimental (gys1^(lox/lox) – mercremer^(+/−)) and control (gys1^(lox/lox) – mercremer^(−/−)) groups respectively. Both groups underwent tamoxifen treatment following their genotype identification.