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. 2016 Feb 29;6:22286. doi: 10.1038/srep22286

Table 1. The summary of cleavage rules considered in this study.

Short notation Enzyme/Reagent Cleavage position(s) Exceptions from the cleavage rule
AFILMV Thermolysin N-terminal side of A, F, I, L, M, V if D or E is N-term to A, F, I, L, M, V
AFYWLIV Proteinase K C-terminal side of A, F, Y, W, L, I, V
D Microwave-assisted formic acid hydrolysis C-terminal side of D
DE Glu C (phosphate) C-terminal side of D or E if P is C-term to D or E, or if E is C-term to D or E
DEK Glu C (phosphate)+Lys C C-terminal side of D, E and K if P is C-term to D or E, or if E is C-term to D or E
DK Asp N/Lys C N-terminal side of D, C-terminal side of K
E Glu C (bicarbonate) C-terminal side of E if P is C-term to E, or if E is C-term to E
FL Pepsin Inline graphic C-terminal side of F, L
FLMWY Chymotrypsin (F/L/M/W/Y) C-terminal side of F, L, M, W, Y if P is C-term to F, L, M, W, Y, if P is N-term to Y
FLWYAEQ Pepsin (pH > 2) C-terminal side of F, L, W, Y, A, E, Q
FYW Chymotrypsin (F/Y/W) C-terminal side of F, Y, W if P is C-term to F, Y, W, if P is N-term to Y
K Lys C C-terminal side of K
KR Trypsin C-terminal side of K or R if P is C-term to K or R
KRFYW Trypsin/Chymotrypsin C-terminal side of K, R, F, Y, W
M CNBr C-terminal side of M
R Arg C C-terminal side of R if P is C-term to R