Table 2. Fold changes (relative intensity) in the abundance of metabolites detected in the LPS-deficient polymyxin-resistant 19606R, relative to the parent strain ATCC 19606.
| Formula | Putative metabolitea | Pathway/metabolism | Fold change | P-value |
|---|---|---|---|---|
| Carbohydrate | ||||
| C5H12O5 | Xylitol | Pentose and glucoronate interconversions | 3.47 | 0.00017 |
| C12H23O14P | Lactose 6-phosphate | Galactose metabolism | 2.14 | 0.0018 |
| C3H6O9P2 | Cyclic 2,3-bisphospho-D-glycerate | Carbohydrate metabolism | –3.01 | 0.0035 |
| Amino acids | ||||
| C7H11O8P | Shikimate 3-phosphate | Phenylalanine, tyrosine, tryptophan biosynthesis | 14.41 | 0.0012 |
| C10H13O10P | 5-O-(1-Carboxyvinyl)-3-phosphoshikimate | Phenylalanine, tyrosine, tryptophan biosynthesis | 11.30 | 0.0079 |
| C8H8O5 | 3,4-Dihydroxymandelate | Tyrosine | 3.03 | 0.00074 |
| C4H6O3 | 2-Methyl-3-oxopropanoate | Valine, leucine and isoleucine degradation | 2.19 | 0.00060 |
| C2H5O5P | Acetyl phosphate | Taurine and hypotaurine | 2.15 | 9.2E-05 |
| C9H8O3 | Phenylpyruvate | Phenylalanine | –2.00 | 5.9E-05 |
| C9H10O4 | 3-(2,3-Dihydroxyphenyl)propanoate | Phenylalanine | –2.03 | 0.0011 |
| C13H15NO6 | 4-Hydroxyphenylacetylglutamic acid | Tyrosine | –2.08 | 0.0077 |
| C7H15NO3 | L-Carnitine | Lysine degradation | –2.57 | 0.00049 |
| C6H6N2O2 | Urocanate | Histidine | –3.75 | 0.0031 |
| C6H10N2O4 | N-Formimino-L-glutamate | Histidine | –24.86 | 0.00095 |
aPutative metabolites, identified by exact mass, with at least 2-fold differences at p value < 0.01 between the polymyxin-resistant 19606R and the polymyxin-susceptible ATCC 19606.