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. Author manuscript; available in PMC: 2016 Feb 29.
Published in final edited form as: Methods Enzymol. 2014 Dec 26;552:185–210. doi: 10.1016/bs.mie.2014.10.007

Table 1.

Working dilutions of the antibodies used in the protocol

Antibody Working dilution
Mouse anti–β–actin (Santa Cruz, sc–47778) 1:5000 in 0.5% BSA/milk
Rabbit anti–Peroxiredoxin–SO2/3 (Abeam, ab 16830) 1:10,000 in 0.5% BSA/milk
Mouse anti–2–Cys Peroxiredoxin (Abeam, ab 16765) 1:1000 in 0.5% BSA/milk

All antibody dilutions are made up in blocking buffer with 0.02% sodium azide and incubated with a blot overnight at 4 °C on a shaking table.