Table 1.
Primers used in this study. Primers orf1 and orf2 [16] were used for the preliminary screening of the clinical samples. The remaining primers were used for the amplification and sequencing of the full-length A32L (825 nt) and B2L (1137 nt) genes of ORFV. The estimated PCR product sizes are presented
| SI No | Primer name | Sequences (5'– 3') | Application | PCR product size (base pair) |
|---|---|---|---|---|
| 1 | Orf1 | CGCAGACGTGGCTGAGTACGT | PCR | 140 |
| 2 | Orf2 | TGAGCTGGTTGGCGCTGTCCT | ||
| 3 | ORFV-A32f-For | CTCCATTTAGAGGCCGTGAG | PCR/Sequencing | 1098 |
| 4 | ORFV-A32f-Rev | CGTGTTATGTGCCATCTTGC | ||
| 5 | ORFV-A32i-For | GGTCGAGACAGCGCTTGA | Sequencing | - |
| 6 | ORFV-A32i-Rev | CGTCTACAACGCCGCCTAC | ||
| 7 | ORFV-B2Lf-For | GACCTTCCGCGCTTTAATTT | PCR/Sequencing | 1210 |
| 8 | ORFV-B2Lf-Rev | CCCGCCTGCTAAAAGACT | ||
| 9 | ORFV-B2Li-For | GTCCGCGTTCTTCCACTC | Sequencing | - |
| 10 | ORFV-B2Li-Rev | GCGGGCGTCAACTACTACA | ||
| 11 | SP6 | ATTTAGGTGACACTATAG | Sequencing | - |
| 12 | T7 | TAATACGACTCACTATAGGG |