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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Enzymol. 2015 Dec 14;570:233–260. doi: 10.1016/bs.mie.2015.10.003

Figure 2.

Figure 2

Construct design of receptor and chemokine samples. A.) Schematic overview of chemokine and receptor constructs. B.) SDS-PAGE of purified ACKR3 showing the effect of promoter and tag placement on the final yield of ACKR3 protein. The doubling and fuzziness of the ACKR3 band is due to glycosylation.