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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Enzymol. 2015 Dec 14;570:233–260. doi: 10.1016/bs.mie.2015.10.003

Figure 4.

Figure 4

Expression of ACKR3/CXCL12 complex. A.) Detection of total receptor expression in co-expressed samples using anti-FLAG FITC antibody in the presence of triton X-100 to a final concentration of 0.0075% (v/v). B.) Detection of chemokine binding to receptor at the cell surface using anti-HA FITC antibody. Co-expression with the receptor gives a large signal increase for CXCL12-HA, indicating specific binding of the chemokine to the receptor.