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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Enzymol. 2015 Dec 14;570:233–260. doi: 10.1016/bs.mie.2015.10.003

Figure 5.

Figure 5

Purification of ACKR3/CXCL12 complex. A.) 10% SDS-PAGE of an bril-ACKR3 fusion protein extracted with the small molecule CCX777 or with a mutant of CXCL12 where KPV at the N-terminus was replaced with LRHQ (LRHQ-CXCL12) (Hanes, et al., 2015). B.) Different stages of purification characterized by SDS-PAGE (10%). C.) 18% SDS-PAGE of a co-expressed and co-purified ACKR3/CXCL12 sample (left lane). ACKR3 expressed alone and purified in complex with a small molecule compound is shown in the right lane. D.) Western blot detecting FLAG-tagged ACKR3 and HA-tagged CXCL12 in samples from C.