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. 2016 Feb 29;6:22198. doi: 10.1038/srep22198

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Copyright © 2016, Macmillan Publishers Limited

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(a) Relative MMP2 mRNA expression and (b) MMP2 gelatinase activity in selected cell lines. Recombinant human MMP2 (rhMMP2 66kDa) is taken as positive control and serum free medium as negative control (c) Relative MMP2 mRNA expression, (d) protein expression and (e) active MMP2 protein quantification (f) MMP2 gelatinase activity and (g) quantification of MMP2 gelatinase activity of empty vector control (EV) and MMP2 knock-down (KD) cells of HT1080, U373 and U87 MMP2-KD compared to control cells. (h) Percentage of invading cells in invasive cell lines HT1080 and U373. Data represents the mean +/− SD of at least 3 independent experiments. (***P < 0.0001, **P < 0.01, *P < 0.05).