Table 1.

Oligonucleotide primers for PCR amplification

Gene	Primer sequence (5′–3′)	Annealing temperature (°C)
GAPDH	Forward: ACTCTGGCAAAGTGGATG Reverse: TCCTGGAAGATGGTGATG	60
Aggrecan	Forward: GAGGTCGTGGTGAAAGGTGT Reverse: GTGTGGATGGGGTACCTGAC	62
COL1A1	Forward: AGGGCCAAGACGAAGACATC Reverse: AGATCACGTCATCGCACAACA	60
COL2A1	Forward: GGATAGACCCCAACCAAGGC Reverse: GCTGCTCCACCAGTTCTTCT	62
MMP-3	Forward: GCCAAGAGATGCTGTTGATG Reverse: AGGTCTGTGAAGGCGTTGTA	65