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. 2015 Oct 15;6(36):39235–39246. doi: 10.18632/oncotarget.5743

Figure 4. Cisplatin triggers cell death by increasing NOX5-L activity through Ca2+ release.

Figure 4

A. Analysis of Ca2+ changes in cisplatin-treated HOP-92 cells by measuring Fluo-4-AM fluorescence (n = 3). B. Measurement of ROS by DCF oxidation in HOP-92 and SK-BR-3 cells. Cells were treated with DMSO, BAPTA-AM, EGTA, or thapsigargin in the presence or absence of cisplatin (n = 3). C. Cell viability assays of SK-BR-3 cells. Cells were treated with DMSO, BAPTA-AM, or EGTA in the presence or absence of cisplatin (n = 3). D. Immunoblots of NOX5-L, p-CREB, and actin from G-361 cells. Cells were treated with DMSO or BAPTA-AM in the presence or absence of cisplatin.