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. 2015 Oct 21;6(36):39247–39261. doi: 10.18632/oncotarget.5746

Figure 6. ERp57 triggers proliferation and cell cycle progression of cancer cells independently of p53.

Figure 6

A. Inducible HCT116 shERp57 and MDA-MB-231 shERp57 were irradiated 48 h after induction of ERp57 knockdown. 24 h after irradiation cell cycle distribution (upper panel) was determined by PI staining and subsequent FACS analysis. In the lower panel fractions of the cells in each cell cycle phase are plotted. Representative data of two independent experiments are shown. B. 4d after knockdown induction HCT116 shERp57 and MDA-MB-231 shERp57 cells were irradiated with 3 Gy. Following treatment cells were reseeded. Cell numbers were determined when the control samples had grown to confluency and normalized to non-induced controls. C. 5 d after knockdown induction, cells were treated with 10 μM etoposide for 4 h. Cell numbers were determined as in (B) Images of representative samples were taken prior to counting.