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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Macromol Biosci. 2015 Aug 13;15(12):1663–1672. doi: 10.1002/mabi.201500192

Figure 7.

Figure 7

(a) Confocal microscopic image of GFP expression 48 h post transfection in ARPE-19 cell line under serum-free and 10 % serum transfection media. Transfection of G4/ECO/pDNA N/N/P ratios 2/1/1, 2/2/1, 2/3/1, 3/1/1, 3/2/1 and 3/3/1 nanoparticles were evaluated. (b) Flow cytometry of GFP expression 48 h post transfection of ARPE-19 cell line under serum-free and 10 % serum transfection media. G4/ECO/pDNA nanoparticles were tested over a range of N/N/P ratios with lipofectamine 2000, ECO/pDNA particles and G4/pDNA particles used as controls.