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. 2015 Oct 14;25(2):189–201. doi: 10.1089/scd.2015.0190

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© Yuan Hong Yu, et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (<http://creativecommons.org/licenses/by-nc/4.0/>) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 3. — Characteristics of LeX/C1qR1-sorted cells. Distribution and sorting gates (R1–R4) of the neurosphere-dissociated cells in combined assays. (A) Negative control for LeX/C1qR1, (B) LeX/C1qR1, (C) negative control for Prom1/C1qR1, (D) Prom1/C1qR1. Four regions (R1: doubly positive; R2 and R3: singly positive; and R4: doubly negative) as well as the unsorted (all live cells) from each combined assay were sorted. (E, F) Bar chart showing the percentage of neurospheres generated per single sorted cell for each subpopulation after 14 days of culture. Data are presented as mean ± SEM from four experiments. *P < 0.05, **P < 0.01, ***P < 0.001. (G) Relative expression of neural stem cell (NSC)-specific genes in each of the FACS-sorted subpopulations from (A) was analyzed by quantitative RT-PCR. The fold change in the gene expression profile was compared with unsorted cells (black bar). RT-PCR, reverse transcription polymerase chain reaction.