FIG. 4.

NSC frequency of LeX/C1qR1-sorted cells. (A–D) Embryo forebrain (E14.5) coronal sections were counterstained with DAPI (blue) (A), nestin (green) (B), and NogoR1 (red) (C). Merged is shown in (D). The boxes show the location of the inserts in (B–D). The inserts show optical zoom of the boxed areas. The signals of the insets have been adjusted to visualize colocalization of LeX and C1qR1. Scale bar = 40 μm. (E) Individual clonal neurospheres were differentiated on coverslips coated with PLL/laminin and scored as follows: tripotent (astrocytes, neurons, and oligodendrocytes), bipotent (astrocytes/neurons or astrocytes/oligodendrocytes), and unipotent (astrocytes only). Data are presented as mean ± SEM from three experiments. *P < 0.05, ***P < 0.001. Antibodies and methods as described in the Materials and Methods section. (F) NSC frequency was calculated by multiplying the absolute neurosphere-forming units by the percentage of multipotent neurospheres.