FIG. 5.

Time-lapse imaging of neurosphere formation. LeX⁺/C1qR1⁺ cells were seeded at clonal density in a 96-well plate and imaged from the single-cell stage for 5 days. (A) Cells forming neurospheres. (B) Cells dying without division. (C) Cells dying after one division and (D) cells dying after two divisions. (E) Quantification of data presented in (A–D). Scale bar = 10 μm (Mean ± SEM; n = 5). (F, G) LeX⁺/C1qR1⁺ cells were seeded at clonal density and left for 24 h. Cells were stained with LeX (or C1qR1), doublets imaged for LeX distribution, and scored for neurosphere formation after 5 days.