Figure 1.
Quantities of vaginal bacteria over time, for all subjects, with depot-medroxyprogesterone acetate (DMPA)-fitted trend lines pre- and post-DMPA. A, Bacterial load by broad-range 16S rRNA gene qPCR. B, Gardnerella vaginalis. C, Lactobacillus iners. Bacterial quantities were log10-transformed. Values below the lower limit of detection were assigned a value of half the lower limit of detection. P-values and estimates were calculated using linear mixed effects models with linear splines pre- and post-DMPA initiation (random intercepts and random slopes post DMPA-initiation).