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EVs from steady state versus apoptotic MSC cultures. MSC was cultured in chemically defined medium for 72 hours and then treated with or without 1 µM staurosporine for 3 hours. The media were then harvested and extracted with CTB or AV, and the extracts analysed for CD9 and actin by western blot. The CD9 and actin signals were scanned and the CD9 signal in each extract was normalized to actin.
