Skip to main content
. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Wiley Interdiscip Rev RNA. 2015 Dec 27;7(2):227–240. doi: 10.1002/wrna.1327

Figure 1. KSRP decreases GAP-43 mRNA stability and axonal outgrowth.

Figure 1

(A) As shown by Bird and coworkers47, addition of recombinant KSRP to S100 extracts from Ksrp -/- mouse brains decreases the half-life of the GAP-43 mRNA. Decay curves show the average results of 3 separate decay experiments fitted with a single rate exponential decay curve. *p<0.05. The effect of KSRP required both the presence of the KH4 domain in the protein and the ARE in the GAP-43 3’ UTR. (B) Overexpression (OE) of KSRP in primary hippocampal neuron cultures impairs axonal elongation. This process is reversed by either KSRP knockdown (KD) or by OE of a GAP-43 mRNA with a 3’ UTR targeting sequence for axonal localization47.