Table 1.

OX130 MAb Blocks CD47 Binding to SIRPα(1)

Protein immobilized	Level of immobilized protein	CD47 binding	CD47 binding post OX130 injection
SIRPα(1)	862	154	22
SIRPα(10)	956	280	247
SIRPβ1(1)	1036	15	2

BIAcore analysis showing response units (RU) of three SIRP proteins immobilized via their CD4 tag. RU values for CD47 passed over the proteins are shown. OX130 MAb was then passed over and CD47 passed over again. Specific binding responses were calculated by subtracting RU obtained for the immobilized control protein rat CD4 domains 3 and 4. The OX130 MAb gives almost complete inhibition of CD47 binding to SIRPα(1) but has no effect on SIRPα(10), to which it does not bind (see Fig. 1). SIRPβ1(1) shows minimal binding of CD47 as expected.