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. Author manuscript; available in PMC: 2016 Feb 29.
Published in final edited form as: Immunomethods. 1992 Dec;1(3):159–168. doi: 10.1016/S1058-6687(05)80012-9

FIG. 2.

FIG. 2

Enumeration of IgM-, IgG-, IgA-, and IgD-producing lymphocytes among EBV-infected B cells. B cells from one subject were infected with EBV and then distributed at varying numbers in microculture wells. After 1 (solid circles), 2 (open circles), 3 (solid triangles), and 4 (open triangles) weeks, the supernatants were tested for their IgM, IgG, IgA, and IgD contents. The fraction of negative wells (wells not containing Ig) of the total wells seeded at each cell concentration was plotted against the number of cells plated per well. Frequency of Ig-secreting cells was calculated using Poisson distribution analysis. The projection on the X axis of the intercept of (---) (fraction of negative wells, 0.37) with each line derived from the experimental data corresponds to the cell dose containing one B lymphocyte producing IgM, IgG, IgA, or IgD.