Figure 2.

The slo^Δ55b mutation affects basal expression from the muscle/tracheal cell promoter but does not affect basal expression from the neural promoters of slo. Relative slo expression levels in wild-type and slo^Δ55b stocks determined by real-time RT-PCR using either C1 primers that detect transcripts from both neural promoters (Panel A) or C2 primers that detect transcripts from the muscle/tracheal cell promoter (Panel B). A) Basal expression of neural promoter specific transcripts is not altered by the slo^Δ55b mutation. B) Basal expression from muscle/tracheal-specific promoter is altered by the slo^Δ55b mutation. Transcript abundance is expressed relative to the wild type. Statistical significance was determined by Student’s t test (n = 3, *P ≤ 0.05). Error bars represent SEM.