Figure 3.
Benzyl alcohol induction of slo does not differ between wild-type and sloΔ55b stocks. Relative slo expression levels in wild-type and sloΔ55b stocks determined by real-time RT-PCR using either C1 primers that detect transcripts from the neural promoters (Panels A–D) or C2 primers that detect transcripts from the muscle/tracheal cell promoter (Panel E–F). A–D) Benzyl alcohol induction of slo neuronal–promoter expression of wild-type and sloΔ55b stocks is similar. E, F) Benzyl alcohol does not induce expression of the muscle/tracheal cell promoter in wild-type or sloΔ55b stocks. Transcript abundance is expressed relative to mock-sedated control animals. Statistical significance was determined by Student’s t test (n = 3, *P ≤ 0.05). Error bars represent SEM.
