Figure 4.
Patterns of histone H4 acetylation across slo transcriptional control region in sloΔ55b homozygotes after benzyl alcohol (BA) sedation. A) Map of slo control region as described in Figure 1A. In panels B-D the fold change of acetylation was determined by chromatin immunoprecipitation followed by real-time PCR using primers specific to the distinct conserved elements depicted as red boxes in (A). Change in acetylation is expressed as the ratio of the acetylation levels in benzyl alcohol-sedated flies and untreated flies. B) H4 acetylation levels at 4 h after benzyl alcohol sedation. C) Acetylation levels at 6 h after benzyl alcohol sedation. D) Acetylation levels at 24 h after benzyl alcohol sedation. Statistical significance was determined by one-way ANOVA with Dunnett’s comparison post tests (n = 3, *P ≤ 0.05) but error bars are SEM.
